Risk-free Guaranteed E. coli Protein Package
from gene synthesis to tagged protein purification

Tag option: His tag, GST tag or Strep tag
Purity: 85% /90% /95%
Quantity: from milligrams to grams

Optional: Endotoxin removal or Antibody production

Phase I: Gene Synthesis & Cloning
- Codon optimization and Gene synthesis
- Sub-cloning into expression vectors
- Plasmid preparation

Phase II: Protein Expression & Purification
- Pilot expression optimization
- Scale up expression
- Purification (affinity purification)
- QC by SDS-PAGE & WB

- Tagged Protein
(Guaranteed purity & quantity)
- 4ug DNA with the gene insert
- CoA: SDS-PAGE & WB data

6-8 weeks

Risk-free Guaranteed E. coli Protein Package
from gene synthesis to tag-free protein purification

Tag option: Tag-free (through tag-free production)*
Purity: 85% /90% /95%
Quantity: from milligrams to grams

Optional: Endotoxin removal

Phase I: Gene Synthesis & Cloning
- Codon optimization and Gene synthesis
- Sub-cloning into expression vectors
- Plasmid preparation

Phase II: Protein Expression & Purification
- Pilot expression optimization
- Scale up expression
- Tag-free purification (SEC and ion exchange)
- QC by SDS-PAGE & MALDI-TOF (optional)

- Tag-free Protein
(Guaranteed purity & quantity)
- 4ug DNA with the gene insert
- CoA: SDS-PAGE & WB data

6-9 weeks